Clonal growth of Blastocystis hominis in soft agar with sodium thioglycollate

  title={Clonal growth of Blastocystis hominis in soft agar with sodium thioglycollate},
  author={S. W. Tan and M. Singh and K. T. Thong and L. C. Ho and K. T. Moe and Xiang Qun Chen and G. C. Ng and E. H. Yap},
  journal={Parasitology Research},
Abstract The present report describes a method for establishment of colonies of Blastocystis hominis from single cells in soft agar. The percentage of colony-forming efficiency (% CFE = number of colonies grown / number of cells inoculated × 100) for the cultures was greatly improved by the addition of sodium thioglycollate. Five human Blastocystis isolates chosen for this study showed no apparent variation in colonial morphology. Isolated colonies were also successfully grown in liquid medium… Expand
Colony growth of Philippine isolates of Blastocystis hominis in simplified, soft agar medium
This is the first report on agar cloning of Blastocystis in a compound medium and analysis of the SSU rDNA genetically established the identity of the clones to be B. hominis. Expand
Blastocystis hominis: A simplified, high-efficiency method for clonal growth on solid agar.
This study provides the first report of clonal growth of B. hominis on agar and a simple, effective method for cloning and expansion of B., hominis cells as colonies on solid agar. Expand
Colony growth as a step towards axenization of Blastocystis isolates
  • G. Ng, K. Tan
  • Biology, Medicine
  • Parasitology Research
  • 1999
Abstract Colonies of Blastocystis from non-axenic cultures grown in agar medium were isolated from bacterial colonies. Axenization of human and reptilian isolates of Blastocystis was achieved usingExpand
Description of a Blastocystis species from Rattus norvegicus
Two isolates (WR1 and WR2) of Blastocystis from laboratory-bred Wistar rats were axenized by a method of colony growth in soft agar combined with antibiotic treatment, resulting in the first report of cyst formation in in vitro culture. Expand
Morphology and reproductive mode of Blastocystis hominis in diarrhea and in vitro
The in vitro culture of Blastocystis hominis in Roswell Park Memorial Institute (RPMI) 1640 medium containing 20% calf serum is described and three distinct morphological forms, vacuolar, granular and amoeboid, were distinguished in faeces and in vitro cultures. Expand
Clonal cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and a Blastocystis sp. established through micromanipulation
Clonal cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and a Blastocystis sp. were established for the first time. Single microbes were successfully isolated from a mixture ofExpand
Encystation—survival of Blastocystis hominis in immunocompetent mice abdomen cavity
Results showed that encystation enable the parasite to avoid the immune attack in competent host and simultaneously decrease the pathogenicity to host. Expand
Blastocystis hominis: origin and significance of vacuolar and granular forms
A rapid transition from uniformly stained to granular and vacuolated forms preceded the organism's death as evidenced by pale staining and Brownian motion in the cell's interior. Expand
Blastocystis hominis: neutral red supravital staining and its application to in vitro drug sensitivity testing
Abstract A simple in vitro drug sensitivity testing system for Blastocystis hominis clinical isolates was developed. Application of supravital staining by neutral red allowed quantitative viabilityExpand
Ultrastructural insights into morphology and reproductive mode of Blastocystis hominis
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Colony formation of Blastocystis hominis in soft agar
This is the first description of a method for growing axenized Blastocystis hominis as colonies in petri dishes containing soft agar, and many cells were shown to possess thin filament-like structures that appeared to stretch across the central vacuole. Expand
Clonal growth of Entamoeba histolytica and other species of Entamoeba in agar.
It was demonstrated in a study with the drug metronidazole that the agar colony method was useful for quantifying cell viability in drug testing, and was dependent upon filling deep vessels with agar to maintain reduced oxygen tension. Expand
Studies of Blastocystis hominis.
  • C. Zierdt
  • Biology, Medicine
  • The Journal of protozoology
  • 1973
SYNOPSIS Blastocystis hominis, grown in Boeck-Drbohlav culture medium, modified by the omission of rice starch and the addition of 20% human serum and mineral oil cover to the Locke's solutionExpand
Clonal growth in Petri dishes of Entamoeba histolytica.
  • D. E. Mueller, W. Petri,
  • Biology, Medicine
  • Transactions of the Royal Society of Tropical Medicine and Hygiene
  • 1995
Colonial morphology of Trichomonas vaginalis in Agar.