Clonal analysis of the glycosylation of immunoglobulin G secreted by murine hybridomas.

@article{Rothman1989ClonalAO,
  title={Clonal analysis of the glycosylation of immunoglobulin G secreted by murine hybridomas.},
  author={Ronald J Rothman and L. Patrick Warren and Johannes F. G. Vliegenthart and K J H{\"a}rd},
  journal={Biochemistry},
  year={1989},
  volume={28 3},
  pages={1377-84}
}
A panel of 10 hybridomas was assembled to assess the influence of various genetic and biological factors upon glycosylation of secreted monoclonal IgG. After exhaustive Pronase digestion of IgG, glycopeptides were characterized chromatographically by apparent size, charge, and concanavalin A (Con A)-Sepharose and Lens culinaris (LcH)-agarose affinity. Six glycosylation phenotypes were found to be common among all clones studied. Despite this phenotypic heterogeneity in glycosylation of IgG… CONTINUE READING

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