Cloacal and buccal swabs are a reliable source of DNA for microsatellite genotyping of reptiles

  title={Cloacal and buccal swabs are a reliable source of DNA for microsatellite genotyping of reptiles},
  author={Hilary C. Miller},
  journal={Conservation Genetics},
In this study, a minimally invasive method for DNA sampling of reptiles and amphibians using cloacal and buccal swabs is described. High molecular weight DNA was isolated from the swabs, which were collected from tuatara (Sphenodon punctatus), and stored in 70% ethanol at room temperature for approximately 1 week. Amplification of mitochondrial and microsatellite DNA loci was successful from both cloacal and buccal swabs, and in all cases the genotypes matched those obtained from blood samples… CONTINUE READING
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