Clinical relevance and therapeutic significance of microRNA-133a expression profiles and functions in malignant osteosarcoma-initiating cells.

Abstract

Novel strategies against treatment-resistant tumor cells remain a challenging but promising therapeutic approach. Despite accumulated evidence suggesting the presence of highly malignant cell populations within tumors, the unsolved issues such as in vivo targeting and clinical relevance remain. Here, we report a preclinical trial based on the identified molecular mechanisms underlying osteosarcoma-initiating cells and their clinical relevance. We identified key microRNAs (miRNAs) that were deregulated in a highly malignant CD133(high) population and found that miR-133a regulated the cell invasion that characterizes a lethal tumor phenotype. Silencing of miR-133a with locked nucleic acid (LNA) reduced cell invasion of this cell population, and systemic administration of LNA along with chemotherapy suppressed lung metastasis and prolonged the survival of osteosarcoma-bearing mice. Furthermore, in a clinical study, high expression levels of CD133 and miR-133a were significantly correlated with poor prognosis, whereas high expression levels of the four miR-133a target genes were correlated with good prognosis. Overall, silencing of miR-133a with concurrent chemotherapy would represent a novel strategy that targets multiple regulatory pathways associated with metastasis of the malignant cell population within osteosarcoma.

DOI: 10.1002/stem.1618
0501002014201520162017
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@article{Fujiwara2014ClinicalRA, title={Clinical relevance and therapeutic significance of microRNA-133a expression profiles and functions in malignant osteosarcoma-initiating cells.}, author={Tomohiro Fujiwara and Takeshi Katsuda and Keitaro Hagiwara and Nobuyoshi Kosaka and Yusuke Yoshioka and Ryou-u Takahashi and Fumitaka Takeshita and Daisuke Kubota and Tadashi Kondo and Hitoshi Ichikawa and Akihiko Yoshida and Eisuke Kobayashi and Akira S. Kawai and Toshifumi Ozaki and Takahiro Ochiya}, journal={Stem cells}, year={2014}, volume={32 4}, pages={959-73} }