Clinical-grade, large-scale, feeder-free expansion of highly active human natural killer cells for adoptive immunotherapy using an automated bioreactor.

@article{Sutlu2010ClinicalgradeLF,
  title={Clinical-grade, large-scale, feeder-free expansion of highly active human natural killer cells for adoptive immunotherapy using an automated bioreactor.},
  author={Tolga Sutlu and Birgitta Stellan and Mari Gilljam and Hern{\'a}n Concha Quezada and Hareth Nahi and G{\"o}sta Gahrton and Evren Alici},
  journal={Cytotherapy},
  year={2010},
  volume={12 8},
  pages={
          1044-55
        }
}
BACKGROUND AIMS Natural killer (NK) cell-based adoptive immunotherapy is a promising approach for the treatment of cancer. Ex vivo expansion and activation of NK cells under good manufacturing practice (GMP) conditions are crucial for facilitating large clinical trials. The goal of this study was to optimize a large-scale, feeder-free, closed system for efficient NK cell expansion. METHODS Peripheral blood mononuclear cells (PBMCs) from healthy donors and myeloma patients were cultured for 21… CONTINUE READING

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