Clinical evaluation of an enzyme-linked immunosorbent assay kit for determination of plasma insulin concentrations in cats.

  title={Clinical evaluation of an enzyme-linked immunosorbent assay kit for determination of plasma insulin concentrations in cats.},
  author={Kyoji Yasuda and Naohito Nishii and Yasunori Ohba and Satoshi Takashima and Mitsuru Takagi and Yuki Touhata and Haruki Shibata and Hitoshi Kitagawa},
  journal={Journal of the Japan Veterinary Medical Association},
1 Citations

Insulin responses to administrations of amino acids and fatty acids in healthy cats.

The intravenous arginine tolerance test may be useful for estimation of insulin responses in cats after intravenous administration of various amino acids and essential fatty acids in clinically healthy adult cats.



Relationships Between Intravenous Glucose Loads, Insulin Responses and Glucose Disappearance Rate1

Estimated from the 3–5′ Δ plasma insulin levels, the early insulin response, which has been previously shown to be derived from a functional pool of stored insulin, increased in a nonlinear manner over the entire dosage range, suggesting that the total insulin output proportionally responds to the size of the glucose dose.

Insulin Sensitivity Decreases with Obesity, and Lean Cats with Low Insulin Sensitivity are at Greatest Risk of Glucose Intolerance with Weight Gain

Results suggest an underlying predisposition for glucose intolerance in some cats, which is exacerbated by obesity, may be more at risk of progressing to overt type 2 diabetes mellitus.

Relationship Between Insulin Response During the Intravenous Glucose Tolerance Test, Rate of Fractional Glucose Removal and the Degree of Insulin Resistance in Normal Adults

The height of the insulin response during an IVGTT has only a modest correlation with the KG, it correlates quite well with the degree of insulin resistance, and significant correlations were found between the early, middle, late and total insulin responses.

Glucose tolerance and insulin response in normal-weight and obese cats.

The impaired glucose tolerance and altered insulin response to glucose infusion in the obese cats was believed to be attributable to deleterious effects of obesity on insulin action and beta-cell responsiveness to stimuli (ie, glucose).

A novel sandwich enzyme-linked immunosorbent assay for feline insulin.

A novel sandwich enzyme-linked immunosorbent assay (ELISA) was established to determine the serum insulin concentrations in domestic cats and will strongly help studies on feline diabetes mellitus.

Acute insulin response to intravenous arginine in nonobese healthy cats.

Findings indicate that the arginine challenge is a more meaningful tool than is the IVGTT for evaluating the insulin secretory capacity in cats.

Determination of reference values for glucose tolerance, insulin tolerance, and insulin sensitivity tests in clinically normal cats.

GTT, ITT, and IST can be performed in cats, using standard protocols, and knowledge of reference values and test variability will enable researchers to better interpret test results for assessment of glucose tolerance, pancreatic beta-cell function, and insulin sensitivity in cats.

Glucose uptake and insulin secretory responses to intravenous glucose loads in the dog.

The pattern of responses indicates that the optimum glucose load is near 600 mg/kg for evaluating normal glucose metabolism in dogs by the IV glucose loading technique.