Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.

@article{Dumont2012ClickmediatedLO,
  title={Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.},
  author={Audrey Dumont and Annie Malleron and Monzer Awwad and Sam Dukan and Boris Vauzeilles},
  journal={Angewandte Chemie},
  year={2012},
  volume={51 13},
  pages={3143-6}
}
Metabolic glycan labeling has recently emerged as a very powerful method for studying cell-surface glycans, which has applications that range from imaging glycans in living multicellular organisms, such as zebrafish or mice, to the identification of metastasis-associated cell-surface sialoglycoproteins. This strategy relies on the cellular biosynthetic machinery assimilating a modified monosaccharide that contains a bioorthogonal chemical reporter. The metabolic incorporation of this reporter… CONTINUE READING
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