Click-electron microscopy for imaging metabolically tagged non-protein biomolecules

  title={Click-electron microscopy for imaging metabolically tagged non-protein biomolecules},
  author={John T. Ngo and Stephen R. Adams and Thomas J. Deerinck and Daniela Boassa and Frances P. Rodriguez-Rivera and Sakina F Palida and Carolyn R. Bertozzi and Mark Ellisman and Roger Tsien},
  journal={Nature chemical biology},
  pages={459 - 465}
Electron microscopy (EM) has long been the main technique to image cell structures with nanometer resolution, but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce “Click-EM,” a labeling technique for correlative light microscopy and EM imaging of non-protein biomolecules. In this approach, metabolic labeling substrates containing bioorthogonal functional groups are provided to cells for incorporation into biopolymers by endogenous… 

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