Click-electron microscopy for imaging metabolically tagged non-protein biomolecules

@inproceedings{Ngo2016ClickelectronMF,
  title={Click-electron microscopy for imaging metabolically tagged non-protein biomolecules},
  author={John Thomas Galderon Ngo and Stephen R Adams and Thomas J. Deerinck and Daniela Boassa and Frances P. Rodriguez-Rivera and Sakina F Palida and Carolyn R Bertozzi and Mark H. Ellisman and Roger Tsien},
  booktitle={Nature chemical biology},
  year={2016}
}
EM has long been the main technique for imaging cell structures with nanometer resolution but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce click-EM, a labeling technique for correlative light microscopy and EM imaging of nonprotein biomolecules. In this approach, metabolic labeling substrates containing bioorthogonal functional groups are provided to cells for incorporation into biopolymers by endogenous biosynthetic machinery… CONTINUE READING
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