Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase

@article{LanyonHogg2015ClickCA,
  title={Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase},
  author={Thomas Lanyon-Hogg and N. Masumoto and George Bodakh and Antonios D. Konitsiotis and Emmanuelle Thinon and U. R. Rodgers and R. Owens and A. Magee and E. Tate},
  journal={Analytical Biochemistry},
  year={2015},
  volume={490},
  pages={66 - 72}
}
Hedgehog signaling is critical for correct embryogenesis and tissue development. However, on maturation, signaling is also found to be aberrantly activated in many cancers. Palmitoylation of the secreted signaling protein sonic hedgehog (Shh) by the enzyme hedgehog acyltransferase (Hhat) is required for functional signaling. To quantify this important posttranslational modification, many in vitro Shh palmitoylation assays employ radiolabeled fatty acids, which have limitations in terms of cost… Expand
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