Cleavage of the star strand facilitates assembly of some microRNAs into Ago2-containing silencing complexes in mammals.

@article{Shin2008CleavageOT,
  title={Cleavage of the star strand facilitates assembly of some microRNAs into Ago2-containing silencing complexes in mammals.},
  author={Chanseok Shin},
  journal={Molecules and cells},
  year={2008},
  volume={26 3},
  pages={308-13}
}
In animals, microRNAs (miRNAs) and small interfering RNAs (siRNAs) repress expression of protein coding genes by assembling distinct RNA-induced silencing complexes (RISCs). It has previously been shown that passenger-strand cleavage is the predominant mechanism when siRNA duplexes are loaded into Argonaute2 (Ago2)-containing RISC, while an unwinding bypass mechanism is favored for miRNA duplexes with mismatches. Here I present experimental data indicating that some mammalian miRNAs are… CONTINUE READING