Cleavage of the 190-kDa subunit of DNA-dependent RNA polymerase I yields small polypeptides capable of degrading DNA.

@article{Szopa1986CleavageOT,
  title={Cleavage of the 190-kDa subunit of DNA-dependent RNA polymerase I yields small polypeptides capable of degrading DNA.},
  author={Jan Szopa and Kathleen M. Rose},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 19},
  pages={9022-8}
}
The lability of RNA polymerase I has been examined by incubation at 37 degrees C. Under the conditions used virtually all enzyme activity was lost after 90 min incubation at this temperature. Concomitant with the decrease in enzyme activity was the disappearance of the 190-kDa enzyme subunit and the production of three small polypeptides of Mr 14,000-19,000. Using immunological methods the small polypeptides were shown to be cleavage products of the 190-kDa subunit. The 190-kDa-derived… CONTINUE READING