Cleavage of supercoiled plasmid DNA by autoantibody Fab fragment: application of the flow linear dichroism technique.

@article{Gololobov1995CleavageOS,
  title={Cleavage of supercoiled plasmid DNA by autoantibody Fab fragment: application of the flow linear dichroism technique.},
  author={G. V. Gololobov and E A Chernova and D V Schourov and Ivan V. Smirnov and Irina A. Kudelina and Alexandre G. Gabibov},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 1},
  pages={254-7}
}
A highly effective method consisting of two affinity chromatography steps and ion-exchange and gel-filtration chromatography steps was developed for purification of autoantibodies from human sera with DNA-hydrolyzing activity. Antibody Fab fragment, which had been purified 130-fold, was shown to catalyze plasmid DNA cleavage. The flow linear dichroism technique was used for quantitative and qualitative studying of supercoiled plasmid DNA cleavage by these autoantibodies in comparison with DNase… CONTINUE READING

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