Cleavage of bacterial flagellin with cyanogen bromide. Antigenic properties of the protein fragments.

@article{Parish1969CleavageOB,
  title={Cleavage of bacterial flagellin with cyanogen bromide. Antigenic properties of the protein fragments.},
  author={Christopher Richard Parish and Richard Wistar and Gordon L. Ada},
  journal={The Biochemical journal},
  year={1969},
  volume={113 3},
  pages={501-6}
}
1. Four polypeptide fragments, obtained by cyanogen bromide treatment of the protein flagellin from Salmonella adelaide, were tested for their antigenic activity by using them as inhibitors in three different assays: bacterial immobilization, haemagglutination of sensitized erythrocytes and quantitative micro precipitation. Immunodiffusion studies were also performed on the protein fragments. 2. Cleavage of the flagellin molecule in this way gave no detectable loss of antigenic determinants… CONTINUE READING