Cleavage of FLICE (caspase-8) by granzyme B during cytotoxic T lymphocyte-induced apoptosis.

  title={Cleavage of FLICE (caspase-8) by granzyme B during cytotoxic T lymphocyte-induced apoptosis.},
  author={Jan Paul Medema and Ren{\'e} E. M. Toes and Carsten Scaffidi and Timothy S. Zheng and Richard A Flavell and Cornelis J M Melief and Marcus E Peter and Rienk Offringa and Peter Heinrich Krammer},
  journal={European journal of immunology},
  volume={27 12},
Cytotoxic T lymphocytes induce apoptosis in target cells through the CD95(APO-1/Fas) and the perforin/granzyme B (GrB) pathway. The exact substrate of GrB in vivo is still unknown, but to induce apoptosis GrB requires the activity of caspases in target cells. We show here that in HeLa target cells induction of apoptosis through the perforin/GrB pathway resulted in minor direct cleavage of CPP32 (caspase-3) by GrB. Most caspase-3 cleavage resulted from activation of an upstream caspase. Moreover… CONTINUE READING
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