Cleanup procedure for determination of aflatoxins in major agricultural commodities by liquid chromatography.

@article{Sobolev2002CleanupPF,
  title={Cleanup procedure for determination of aflatoxins in major agricultural commodities by liquid chromatography.},
  author={Victor Sobolev and Joe W. Dorner},
  journal={Journal of AOAC International},
  year={2002},
  volume={85 3},
  pages={
          642-5
        }
}
A simple, fast, reliable, and inexpensive chemical cleanup procedure was developed for quantitation of aflatoxins in major important agricultural commodities by liquid chromatography (LC). Aflatoxins were extracted from a ground sample with methanol-water (80 + 20, v/v), and after a single cleanup step on a minicolumn packed with basic aluminum oxide, they were quantitated by LC equipped with a C18 column, photochemical reactor, and fluorescence detector. Water-methanol-1-butanol (1,400 + 720… CONTINUE READING
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