Clathrin-coated vesicle subtypes in mammalian brain tissue: detection of polypeptide heterogeneity by immunoprecipitation with monoclonal antibodies.


A panel of monoclonal antibodies (mAbs) was developed to identify polypeptides sorted in subtypes of brain coated vesicles (CVs) and to separate these by immunoprecipitation. The corresponding antigen of some of the mAbs elicited by CV components was present also in synaptosomal plasma membrane, synaptic vesicles, or microsomes. On immunoblots the mAbs… (More)


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