Classification and identification of propionibacteria based on ribosomal RNA genes and PCR.

@article{Dasen1998ClassificationAI,
  title={Classification and identification of propionibacteria based on ribosomal RNA genes and PCR.},
  author={Gottfried Dasen and Jana Smutny and Michael Teuber and L{\'e}o Meile},
  journal={Systematic and applied microbiology},
  year={1998},
  volume={21 2},
  pages={251-9}
}
A rapid method was developed to differentiate the genus Propionibacterium from other genera by using a modified multiplex-PCR (MPCR) approach. Three 16S rRNA-targeted oligonucleotide primers were designed to amplify simultaneously two DNA-fragments in the MPCR assay. The universal primer pair bak11w and bak4 (corresponding to the E. coli 16S rRNA positions 8-25 and 1522-1540, respectively) was used in combination with the primer pair bak4 and gd1 (5'-TGCTTTCGATACGGGTTGAC-3'). The later sequence… CONTINUE READING

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