Classical swine fever virus: a second ring test to evaluate RT-PCR detection methods.

@article{Paton2000ClassicalSF,
  title={Classical swine fever virus: a second ring test to evaluate RT-PCR detection methods.},
  author={David James Paton and Adrian McGoldrick and Emmanuelle Bensaude and S{\'a}nd{\'o}r Bel{\'a}k and Ch Mittelholzer and Frank Koenen and Hans Vanderhallen and Irene Greiser-Wilke and Holger Scheibner and T Stadejek and Martin Hofmann and Barbara Thuer},
  journal={Veterinary microbiology},
  year={2000},
  volume={77 1-2},
  pages={71-81}
}
Six laboratories participated in a study to compare the sensitivity and specificity of RT-PCR tests for the detection of classical swine fever virus (CSFV). Sets of coded samples were prepared by serial dilution of positive samples and then distributed to each of the laboratories. One set comprised 25 samples of random primed cDNA, synthesised from viral RNA representative of different pestiviruses. The other set comprised samples of blood and serum obtained from virus-free or CSFV-infected… CONTINUE READING

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