Class-selective drug detection: fluorescently-labeled calmodulin as the biorecognition element for phenothiazines and tricyclic antidepressants.

@article{Douglass2002ClassselectiveDD,
  title={Class-selective drug detection: fluorescently-labeled calmodulin as the biorecognition element for phenothiazines and tricyclic antidepressants.},
  author={Phillip M Douglass and Lyndon L E Salins and Emre Dikici and Sylvia Daunert},
  journal={Bioconjugate chemistry},
  year={2002},
  volume={13 6},
  pages={1186-92}
}
A small-scale, homogeneous, rapid sensing system for phenothiazines and tricyclic antidepressants (TCAs) has been developed by employing fluorescently labeled mutant calmodulin (CaM) as the recognition element. A calmodulin mutant containing a unique cysteine residue at position 109 on the protein was expressed in Escherichia coli. Following purification… CONTINUE READING