Class Switch Recombination and Hypermutation Require Activation-Induced Cytidine Deaminase (AID), a Potential RNA Editing Enzyme

@article{Muramatsu2000ClassSR,
  title={Class Switch Recombination and Hypermutation Require Activation-Induced Cytidine Deaminase (AID), a Potential RNA Editing Enzyme},
  author={Masamichi Muramatsu and Kazuo Kinoshita and Sidonia Fagarasan and Shu-ichi Yamada and Yoichi Shinkai and Tasuku Honjo},
  journal={Cell},
  year={2000},
  volume={102},
  pages={553-563}
}
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Negative regulation of activation-induced cytidine deaminase in B cells.
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    Proceedings of the National Academy of Sciences of the United States of America
  • 2006
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There is an intricate interplay between faithful DNA repair and mutagenic DNA repair during somatic hypermutation, in that some proteins from accurate repair pathways are also involved in mutagenesis.
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination
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It is concluded that AID has a minor impact, if any, on the overall frequency of meiotic recombination, and the frequency of recombination in the female germline was greater than in male germline.
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Activation-Induced Cytidine Deaminase (AID) Deficiency Causes the Autosomal Recessive Form of the Hyper-IgM Syndrome (HIGM2)
Specific Expression of Activation-induced Cytidine Deaminase (AID), a Novel Member of the RNA-editing Deaminase Family in Germinal Center B Cells*
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Findings suggest that AID is a new member of the RNA-editing deaminase family and may play a role in genetic events in the germinal center B cell.
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It is shown that cultures of purified murine and human B cells, stimulated only by CD40 receptor engagement, possess a potent switch recombinations activity and the efficiency of switch recombination with SSRs resembles that seen for endogenous C(H) class switching.
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It is found that the extents of methylation and the amounts of germline transcripts do not necessarily correlate with the efficiency of recombination in induced CH12F3 cells, leading to the proposal that switch recombination can be separated into at least two phases.
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TLDR
Recombination to a particular switch region was abolished in mice that were altered to lack sequences that are 5' to the s gamma 1 region, which directly implicates the functional importance of 5' switch region flanking sequences in the control of class switch recombination.
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TLDR
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Specificity of immunoglobulin heavy chain switch correlates with activity of germline heavy chain genes prior to switching.
TLDR
Results suggest that I.29 cells switch specifically to IgA, IgE or IgG2a due to the activation of the corresponding H chain constant region genes in IgM+ cells prior to the actual switch recombination event.
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