Class I and class II lysyl‐tRNA synthetase mutants and the genetic encoding of pyrrolysine in Methanosarcina spp.

@article{Mahapatra2007ClassIA,
  title={Class I and class II lysyl‐tRNA synthetase mutants and the genetic encoding of pyrrolysine in Methanosarcina spp.},
  author={Anirban Mahapatra and Gayathri Rajaram Srinivasan and Kerstin Richter and Andrew Meyer and Tanja Lienard and Jun Kai Zhang and Gang Zhao and Patrick T. Kang and Michael K. Chan and Gerhard Gottschalk and William W. Metcalf and Joseph Adrian Krzycki},
  journal={Molecular Microbiology},
  year={2007},
  volume={64}
}
Methanosarcina spp. begin methanogenesis from methylamines with methyltransferases made via the translation of UAG as pyrrolysine. In vitro evidence indicates two possible routes to pyrrolysyl‐tRNAPyl. PylS ligates pyrrolysine to tRNAPyl. Alternatively, class I and class II lysyl‐tRNA synthetases (LysRS1 and LysRS2) together form lysyl‐tRNAPyl, a potential intermediate to pyrrolysyl‐tRNAPyl. The unusual possession of both LysRS1 and LysRS2 by Methanosarcina spp. may also reflect differences in… 

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