Citritase, the citrate-splitting enzyme from Escherichia coli. II. Reaction mechanisms.


Spectrophotometric Measurements of Enzymatic Activity-The enzymatic activities of aconitase and isocitric, Lu-ketoglutaric, malic, lactic, and glutamic dehydrogenases were followed in the Beckman DU spectrophotometer in the presence of either triphosphopyridine or diphosphopyridine nucleotide and the requisite substrates. Fumarase activity was detected by… (More)


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