Citrate synthase from the hyperthermophilic Archaeon, Pyrococcus furiosus.

  title={Citrate synthase from the hyperthermophilic Archaeon, Pyrococcus furiosus.},
  author={J M Muir and Robyn J Russell and David W. Hough and M. Danson},
  journal={Protein engineering},
  volume={8 6},
The gene encoding the enzyme citrate synthase has been cloned and sequenced from the hyperthermophilic Archaeon Pyrococcus furiosus, and the derived amino acid sequence has been phylogenetically compared with citrate synthases from archaeal, bacterial and eukaryal organisms. The gene has been over-expressed in Escherichia coli to produce an active enzyme that has then been characterized with respect to its kinetic, oligomeric and hyperthermostable properties. A structurally-based sequence… CONTINUE READING


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