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Production of malignancy in vitro. 4. The mouse fibroblast cultures and changes seen in the living cells. J. This paper describes one of the earliest successful attempts to produce "cancer in a test-tube." Fibroblasts derived from the subcutaneous adipose connective tissue of a C 3 H mouse were treated in vitro with 20-methylcholanthreue. Injection of the… (More)

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