Cisplatin induces apoptosis via upregulating Wrap53 in U-2OS osteosarcoma cells.

@article{Yuan2011CisplatinIA,
  title={Cisplatin induces apoptosis via upregulating Wrap53 in U-2OS osteosarcoma cells.},
  author={Jian-Min Yuan and Xue-Dong Li and Zhao-yong Liu and Guo-qing Hou and Jian-Hui Kang and Dong-yang Huang and Shi-xin Du},
  journal={Asian Pacific journal of cancer prevention : APJCP},
  year={2011},
  volume={12 12},
  pages={3465-9}
}
Wrap531α, a newly identified natural antisense transcript of p53, can regulate p53 expression upon DNA damage. We sought to investigate changes in Wrap53 and p53 levels in an osteosarcoma cell line (U-2OS) exposed to cisplatin and to study apoptosis before and after knockdown of Wrap53. Our RT-PCR analysis showed a dose- dependent 3 to 40-fold increase in Wrap53 mRNA transcript levels in U-2OS exposed to 5 to 20 μM cisplatin. An approximate 2-fold increase was also observed in transcript levels… CONTINUE READING