Circulating tumour cell RNA characterisation from colorectal cancer patient blood after inertial microfluidic enrichment

@inproceedings{Winter2019CirculatingTC,
  title={Circulating tumour cell RNA characterisation from colorectal cancer patient blood after inertial microfluidic enrichment},
  author={Marnie Winter and Zhen Cai and Katharina Schroecksnadel Christiana Winkler and Kristen Ren{\'e}e Georgiou and Daniel J. Inglis and Tina C. Lavranos and Meysam Rezaei and Majid Ebrahimi Warkiani and Benjamin Thierry},
  booktitle={MethodsX},
  year={2019}
}
The detection and molecular analysis of circulating tumour cells (CTCs) potentially provides a significant insight to the characterisation of disease, stage of progression and therapeutic options for cancer patients. Following on from the protocol by Warkiani et al. 2016, which describes a method of enriching CTCs from cancer patient blood with inertial microfluidics, we describe a method to measure the CTC RNA expression in the enriched fraction using droplet digital PCR and compare transcript… CONTINUE READING

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