Circulating miR-221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression.

@article{Pu2010CirculatingMD,
  title={Circulating miR-221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression.},
  author={Xing-xiang Pu and Guo-liang Huang and Hong-qiang Guo and Cheng-cheng Guo and Haoran Li and Shen Ye and Suxia Ling and Li Jiang and Ying Tian and Tongyu Lin},
  journal={Journal of gastroenterology and hepatology},
  year={2010},
  volume={25 10},
  pages={1674-80}
}
BACKGROUND AND AIM Circulating miRNAs exist in serum and plasma and they can be used as a potential noninvasive molecular marker for colorectal cancer (CRC) diagnosis. The present study was to test the availability of direct amplification of miRNAs from plasma without RNA extraction, and to evaluate its clinical application value in CRC. METHODS Plasma miR-21, miR-221 and miR-222 levels were determined in 103 CRC patients and 37 healthy normal controls by quantitative reverse transcription… CONTINUE READING
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