Circadian rhythms in Neurospora crassa: membrane composition of a mutant defective in temperature compensation.

@article{Cot1987CircadianRI,
  title={Circadian rhythms in Neurospora crassa: membrane composition of a mutant defective in temperature compensation.},
  author={G. Cot{\'e} and S. Brody},
  journal={Biochimica et biophysica acta},
  year={1987},
  volume={898 1},
  pages={
          23-36
        }
}
The cel mutant of Neurospora, partially blocked in fatty acid synthesis and lacking temperature compensation of its circadian rhythm below 22 degrees C, had a phospholipid fatty acid composition in liquid shaker culture distinctly different from that of a cel+ control strain. During growth, cel+ exhibited a reproducible increase in its linoleic acid level from about 32 to a plateau at 63 mol%, and a corresponding decrease in its linolenic acid level from about 40 to a plateau at 10 mol%. The… Expand
Circadian rhythms in Neurospora crassa: a clock mutant, prd-1, is altered in membrane fatty acid composition.
TLDR
It is proposed that the prd-1 mutation leads to altered membrane homeostasis, which in turn affects circadian rhythmicity because some or all components of the rhythm-generating system are membrane-localized. Expand
Conversion of palmitate to unsaturated fatty acids differs in a Neurospora crassa mutant with impaired fatty acid synthase activity
TLDR
The Neurospora crassa cel (fatty acid chain elongation) mutant has impaired fatty acid synthase activity, and the Δ15-desaturase activity and elongation activity on 18-carbon polyunsaturated fatty acids are higher for cel than wild-type. Expand
Low-temperature-induced adaptations in fatty acid metabolism of Acanthamoeba castellanii cultures of different ages: relationship to changes in cell division, oxygen uptake and phagocytotic activity
TLDR
A role of membrane lipid unsaturation in the control of phagocytotic activity of A. castellanii is suggested and a rapid and marked increase in the synthesis of polyunsaturated fatty acids is expected following chilling of early- and mid-exponential-phase cultures. Expand
Characterization of Neurospora crassa mutants isolated following repeat-induced point mutation of the beta subunit of fatty acid synthase
TLDR
These mutants are phenotypically less leaky than cel-1, suggesting a low but strict requirement for fatty acid synthesis during sexual development, and inhibition of fatty acid biosynthesis results in a relative increase in both fatty acid desaturation and elongation activity. Expand
period-1 encodes an ATP-dependent RNA helicase that influences nutritional compensation of the Neurospora circadian clock
TLDR
period-1 (prd-1), previously identified in a genetic screen in the filamentous fungus Neurospora, is shown to encode a protein (an ATP-dependent RNA helicase) that is closely related to yeast and human proteins involved in RNA metabolism and transcription, suggesting that normal clock regulation in response to nutrition is altered. Expand
Circadian rhythms in Neurospora crassa: lipid deficiencies restore robust rhythmicity to null frequency and white-collar mutants.
  • P. Lakin-Thomas, S. Brody
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2000
TLDR
Two mutants, cel (chain-elongation) and chol-1 (choline-requirer), which are defective in lipid synthesis and affect the period and temperature compensation of the rhythm are investigated, and it is found that these double mutant strains are robustly rhythmic when assayed under lipid-deficient conditions, indicating that free-running rhythmicity does not require the frq, wC-1, or wc-2 gene products. Expand
Novel Neurospora crassa mutants with altered synthesis of polyunsaturated fatty acids
Five new mutants of Neurospora crassa that require supplementation with unsaturated fatty acids have been isolated. The mutants, designated pfa, are impaired in the synthesis of the polyunsaturatedExpand
Metabolism of ricinoleate by Neurospora crassa
TLDR
Examination of the metabolism and physiological effects of ricinoleate on lipid biosynthesis and growth of N. crassa found it inhibited growth and reduced levels of phospholipids and of 2-hydroxy fatty acids in glycolipids, but led to increased lipid accumulation on a mass basis. Expand
Effect of growth temperature on lipid fatty acids of four fungi (Aspergillus niger, Neurospora crassa, Penicillium chrysogenum, andTrichoderma reesei)
TLDR
Large differences in the effects of growth temperature on mycelial fatty acids were observed among various fungal species, however, the similarities found may indicate common regulatory mechanisms causing the responses. Expand
Closely watched clocks: molecular analysis of circadian rhythms in Neurospora and Drosophila.
  • J. Dunlap
  • Biology, Medicine
  • Trends in genetics : TIG
  • 1990
TLDR
The recent identification and molecular analysis of clock-controlled genes is revealing how biological clocks control gene expression, and may pave the way for the isolation of novel 'clock genes' in the future. Expand
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References

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TLDR
The fatty acid composition of the total lipids, phospholipids and neutral lipids of log-phase shaker cultures of the bd (band) strain of Neurospora crassa, were measured following a temperature increase from 22 to 40 degrees C, indicating that there was little loss of 18:3 from the mycelia by beta-oxidation, or by conversion to other fatty acids. Expand
Circadian rhythms in Neurospora crassa: effects of unsaturated fatty acids
TLDR
Employing a fatty acid-requiring strain (bd csp cel) of Neurospora crassa, the 21.5-h period of the circadian spore-forming rhythm was manipulated by fatty acid supplementation, which lengthened the period to 26, 40, or 33 h, respectively. Expand
Effects of temperature acclimation on Neurospora phospholipids. Fatty acid desaturation appears to be a key element in modifying phospholipid fluid properties.
TLDR
The data are consistent with the idea that phospholipid composition in Neurospora is under strict control and suggest that membrane fluidity is regulated with respect to growth temperature through changes in membrane lipid composition. Expand
Saturated Fatty Acid Requirer of Neurospora crassa
TLDR
The use of a spin-label fatty acid reveals that hyphae produced by cel during a slow basal level of growth have lipids that reflect a relatively rigid state of viscosity compared to wild type. Expand
The effects of temperature acclimation on membrane sterols and phospholipids of Neurospora crassa.
TLDR
Total sterol and phospholipid biosynthetic rates showed parallel reductions in cultures acclimating to a shift from 37 to 15 degrees C growth conditions, indicating an increase in the conversion rate of sterols to sterol esters at the lower temperature. Expand
Circadian nature of a rhythm expressed by an invertaseless strain of Neurospora crassa.
TLDR
A new strain of Neurospora crassa which exhibits a rhythm of conidiation when growing along an agar surface in a growth tube is described and is compared to 2 other strains of neurospora which also express a rhythm, patch and clock. Expand
Genetic Determinants of Circadian Rhythmicity in Neurospora
Timex, a strain of Neurospora crassa which exhibits a circadian rhythm of conidia formation in growth-tube cultures, has been found to differ from wild-type strains by two genes. One gene, inv, isExpand
Isolation of circadian clock mutants of Neurospora crassa.
TLDR
Three mutants of Neurospora crassa have been isolated which have altered period lengths of their circadian rhythm of conidiation, and it is suggested that these mutants represent alterations in the basic timing mechanism of the circadian clock of neurospora. Expand
Glycerophospholipid variation in choline and inositol auxotrophs of Neurospora crassa. Internal compensation among zwitterionic and anionic species.
TLDR
The data suggest the existence of an internal compensation mechanism, the net effect of which is maintenance of a fairly constant contribution by the phospholipid components to the over-all membrane charge. Expand
Temperature-induced modifications of glycosphingolipids in plasma membranes of Neurospora crassa.
TLDR
Fluorescence polarization studies indicate that the observed lipid modifications in 15 degrees C plasma membranes act to modulate bulk fluidity of the plasma-membrane lipids with respect to growth temperature, suggesting that coordinate modulation of glycosphingolipid, phospholipids and sterol content may be involved in regulation of plasma- Membrane fluid properties during temperature acclimation. Expand
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