Circadian expression and induction by wounding of tobacco genes for cysteine proteinase

@article{Linthorst1993CircadianEA,
  title={Circadian expression and induction by wounding of tobacco genes for cysteine proteinase},
  author={Huub J. M. Linthorst and Chris van der Does and Frans Th. Brederode and John F. Bol},
  journal={Plant Molecular Biology},
  year={1993},
  volume={21},
  pages={685-694}
}
Two sets of clones were isolated from a tobacco cDNA library, utilizing as a probe a PCR fragment obtained from tomato cDNA using a degenerate primer based on the sequence of tomato systemin. Contrary to expectation, the clones did not correspond to tobacco homologues of tomato pro-systemin. However, the cDNAs encoded two highly similar proteins with extensive structural homology to cysteine proteinases from a wide range of plant and animal species. Northern blot analyses showed that in… CONTINUE READING
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