Circadian Gene Expression in Individual Fibroblasts Cell-Autonomous and Self-Sustained Oscillators Pass Time to Daughter Cells
@article{Nagoshi2004CircadianGE, title={Circadian Gene Expression in Individual Fibroblasts Cell-Autonomous and Self-Sustained Oscillators Pass Time to Daughter Cells}, author={Emi Nagoshi and Camille Saini and Christoph Bauer and Thierry Laroche and F{\'e}lix Naef and Ueli Schibler}, journal={Cell}, year={2004}, volume={119}, pages={693-705} }
934 Citations
Monitoring cell-autonomous circadian clock rhythms of gene expression using luciferase bioluminescence reporters.
- BiologyJournal of visualized experiments : JoVE
- 2012
A stable transduction protocol using lentivirus-mediated gene delivery and lentiviral vector system is described, superior to traditional methods such as transient transfection and germline transmission because of its efficiency and versatility.
System-Driven and Oscillator-Dependent Circadian Transcription in Mice with a Conditionally Active Liver Clock
- BiologyPLoS biology
- 2007
The finding that rhythmic mPer2 expression can be driven by both systemic cues and local oscillators suggests a plausible mechanism for the phase entrainment of subsidiary clocks in peripheral organs.
Regulation of circadian gene expression in liver by systemic signals and hepatocyte oscillators.
- BiologyCold Spring Harbor symposia on quantitative biology
- 2007
A transgenic mouse strain in which hepatocyte clocks are only operative when the tetracycline analog doxycycline is added to the food or drinking water is developed, which predicts that circadian gene expression in peripheral organs can be influenced either by systemic signals emanating from the SCN master clock, local oscillators, or both.
Molecular Links between the Circadian Clock and the Cell Cycle.
- BiologyJournal of molecular biology
- 2020
Evaluating the Autonomy of the Drosophila Circadian Clock in Dissociated Neuronal Culture
- BiologyFront. Cell. Neurosci.
- 2017
Drosophila clocks are weak oscillators that need to be in the intact circadian circuit to generate robust 24-h rhythms, and the negative feedback loop of the molecular clock is incomplete in isolated clock neurons.
Circadian-independent cell mitosis in immortalized fibroblasts
- BiologyProceedings of the National Academy of Sciences
- 2010
It is found that there was no consistent phase relationship between the circadian and cell cycles, and that the cell-cycle rhythm was not temperature-compensated in rat-1 fibroblasts, which suggests that the circadian system does not regulate theCell mitosis rhythm in immortalized rat- 1 fibro Blasts, inconsistent with numerous studies that suggest that cell mitosis is regulated by the circadianSystem.
Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro
- BiologyProceedings of the National Academy of Sciences
- 2010
It is shown that the circadian bioluminescence rhythm is not detected in the mouse embryonic stem (ES) cells, and that the ES cells likely lack TTFL regulation for clock gene expression, and demonstrated that an intrinsic program controls the formation of the circadian oscillator during the differentiation process of ES cells in vitro.
Molecular components of the mammalian circadian clock.
- BiologyHuman molecular genetics
- 2006
The general mechanisms of the circadian clockwork are reviewed, recent findings that elucidate tissue-specific expression patterns of the clock genes are described and the importance of circadian regulation in peripheral tissues for an organism's overall well-being is addressed.
CLOCK GENES OF MAMMALIAN CELLS: PRACTICAL IMPLICATIONS IN TISSUE CULTURE
- Biology
- 2005
Isolation methods of fragile cell phenotypes may benefit from research on the biological clocks to design improved tissue culture media and new bioassays to diagnose pernicious consequences for health of circadian rhythm alterations.
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