Cigarette smoke induced genotoxicity and respiratory tract pathology: evidence to support reduced exposure time and animal numbers in tobacco product testing

@inproceedings{Dalrymple2016CigaretteSI,
  title={Cigarette smoke induced genotoxicity and respiratory tract pathology: evidence to support reduced exposure time and animal numbers in tobacco product testing},
  author={Annette Dalrymple and Patricia Ordonez and David Thorne and David Q. Walker and Oscar M. Camacho and Ansgar Buettner and Deborah Dillon and Clive Meredith},
  booktitle={Inhalation toxicology},
  year={2016}
}
Many laboratories are working to develop in vitro models that will replace in vivo tests, but occasionally there remains a regulatory expectation of some in vivo testing. Historically, cigarettes have been tested in vivo for 90 days. Recently, methods to reduce and refine animal use have been explored. This study investigated the potential of reducing animal cigarette smoke (CS) exposure to 3 or 6 weeks, and the feasibility of separate lung lobes for histopathology or the Comet assay. Rats were… CONTINUE READING