Chymase cleavage of stem cell factor yields a bioactive, soluble product.

  title={Chymase cleavage of stem cell factor yields a bioactive, soluble product.},
  author={B. Jack Longley and Lynda Tyrrell and Yuliang Ma and David A Williams and Ruth Halaban and Keith Langley and H S Sharon Lu and Norman M. Schechter},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={94 17},
Stem cell factor (SCF) is produced by stromal cells as a membrane-bound molecule, which may be proteolytically cleaved at a site close to the membrane to produce a soluble bioactive form. The proteases producing this cleavage are unknown. In this study, we demonstrate that human mast cell chymase, a chymotrypsin-like protease, cleaves SCF at a novel site. Cleavage is at the peptide bond between Phe-158 and Met-159, which are encoded by exon 6 of the SCF gene. This cleavage results in a soluble… CONTINUE READING
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