Chronic intragastric alcohol exposure causes hypoxia and oxidative stress in the rat pancreas.

@article{McKim2003ChronicIA,
  title={Chronic intragastric alcohol exposure causes hypoxia and oxidative stress in the rat pancreas.},
  author={Stephen E McKim and Takehiko Uesugi and James A. Raleigh and Craig J. McClain and Gavin E. Arteel},
  journal={Archives of biochemistry and biophysics},
  year={2003},
  volume={417 1},
  pages={
          34-43
        }
}
The effect of chronic enteral ethanol on pancreatic hypoxia was investigated using the hypoxia marker, pimonidazole. Male Wistar rats were fed an ethanol-containing diet for 3 weeks using an enteral model shown to cause pancreatic damage; pimonidazole (120 mg/kg i.v.) was injected 1h before sacrifice. Pimonidazole and 4-hydroxynonenal (an index of lipid peroxidation) adducts were detected immunochemically. Breathing air with low oxygen content (8% O(2)) for 1h increased pimonidazole adduct… 
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