Chronic exposure to sublethal doses of radiation mimetic Zeocin selects for clones deficient in homologous recombination.

@article{Delacte2007ChronicET,
  title={Chronic exposure to sublethal doses of radiation mimetic Zeocin selects for clones deficient in homologous recombination.},
  author={Fabien Delac{\^o}te and Ludovic Deriano and Sarah R. Lambert and Pascale Bertrand and Yannick Saintigny and Bernard S Lopez},
  journal={Mutation research},
  year={2007},
  volume={615 1-2},
  pages={125-33}
}
DNA double-strand breaks (DSBs) are highly toxic lesions leading to genome variability/instability. The balance between homologous recombination (HR) and non-homologous end-joining (NHEJ), two alternative DSB repair systems, is essential to ensure genome maintenance in mammalian cells. Here, we transfected CHO hamster cells with the pcDNA3.1/Zeo plasmid, and selected transfectants with Zeocin, a bleomycin analog which produces DSBs. Despite the presence of a Zeocin resistance gene in pcDNA3.1… CONTINUE READING