Chronic exposure to free fatty acids or high glucose induces apoptosis in rat pancreatic islets: possible role of oxidative stress.

@article{Piro2002ChronicET,
  title={Chronic exposure to free fatty acids or high glucose induces apoptosis in rat pancreatic islets: possible role of oxidative stress.},
  author={Salvatore Piro and Marcello Anello and Cinzia Di Pietro and Maria Natalia Lizzio and Giovanni Patan{\'e} and Agata Maria Rabuazzo and Riccardo Vigneri and Michele Purrello and Francesco Purrello},
  journal={Metabolism: clinical and experimental},
  year={2002},
  volume={51 10},
  pages={1340-7}
}
We investigated the effect of a chronic exposure to high levels of free fatty acid (FFA; 2 mmol/L oleate/palmitate 2:1) or glucose (16.7 mmol/L) on islet cell apoptosis. Apoptosis was detected using 4 different methods: (1) cell staining with annexin-V fluorescien isothiocyanate (FITC) conjugate and propidium iodide (PI); (2) quantification of cytoplasmatic DNA fragments by an enzyme-linked immunosorbent assay (ELISA); (3) assay of caspase 3 activity; and (4) TdT-mediated dUTP nick-end labeling… CONTINUE READING