Chronic exposure to alcohol alters network activity and morphology of cultured hippocampal neurons.

Abstract

The effects of chronic exposure to moderate concentrations of ethanol were studied in cultured hippocampal neurons. Network activity, assessed by imaging of [Ca(2+)]i variations, was markedly suppressed following 5 days of exposure to 0.25-1% ethanol. The reduced activity was sustained following extensive washout of ethanol, but the activity recovered by blockade of inhibition with bicuculline. This reduction of network activity was associated with a reduction in rates of mEPSCs, but not in a change in inhibitory synaptic activity. Chronic exposure to ethanol caused a significant reduction in the density of mature dendritic spines, without an effect on dendritic length or arborization. These results indicate that chronic exposure to ethanol causes a reduction in excitatory network drive in hippocampal neurons adding another dimension to the chronic effects of alcohol abuse.

DOI: 10.1016/j.neuro.2015.01.005

Cite this paper

@article{Korkotian2015ChronicET, title={Chronic exposure to alcohol alters network activity and morphology of cultured hippocampal neurons.}, author={Eduard Korkotian and Alena Botalova and Tatiana F. Odegova and Menahem Segal}, journal={Neurotoxicology}, year={2015}, volume={47}, pages={62-71} }