Chronic Cellular Imaging of Entire Cortical Columns in Awake Mice Using Microprisms

Abstract

Two-photon imaging of cortical neurons in vivo has provided unique insights into the structure, function, and plasticity of cortical networks, but this method does not currently allow simultaneous imaging of neurons in the superficial and deepest cortical layers. Here, we describe a simple modification that enables simultaneous, long-term imaging of all… (More)
DOI: 10.1016/j.neuron.2013.07.052

Topics

6 Figures and Tables