Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends

@article{Gorbsky1987ChromosomesMP,
  title={Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends},
  author={Gary J Gorbsky and Paul Sammak and G G Borisy},
  journal={The Journal of Cell Biology},
  year={1987},
  volume={104},
  pages={9 - 18}
}
During the movement of chromosomes in anaphase, microtubules that extend between the kinetochores and the poles shorten. We sought to determine where subunits are lost from these microtubules during their shortening. Prophase or prometaphase cells on coverslips were injected with fluoresceinated tubulin and allowed to progress through mitosis. Immediately after the onset of anaphase, a bar-shaped beam of laser light was used to mark a domain on the kinetochore fibers by photobleaching a band… CONTINUE READING