Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius.

  title={Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius.},
  author={Iain G. Duggin and Simon A McCallum and Stephen D Bell},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={105 43},
The "baby machine" provides a means of generating synchronized cultures of minimally perturbed cells. We describe the use of this technique to establish the key cell-cycle parameters of hyperthermophilic archaea of the genus Sulfolobus. The 3 DNA replication origins of Sulfolobus acidocaldarius were mapped by 2D gel analysis to near 0 (oriC2), 579 (oriC1), and 1,197 kb (oriC3) on the 2,226-kb circular genome, and we present a direct demonstration of their activity within the first few minutes… CONTINUE READING