Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes.

@article{Tanksley1995ChromosomeLA,
  title={Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes.},
  author={Steven D. Tanksley and Martin W. Ganal and Gregory B. Martin},
  journal={Trends in genetics : TIG},
  year={1995},
  volume={11 2},
  pages={63-8}
}
The original concept behind map-based or positional cloning was to find a DNA marker linked to a gene of interest, and then to 'walk' to the gene via overlapping clones (e.g. cosmids or YACs). While chromosome walking is straightforward in organisms with small genomes, it is difficult to apply in most plant species, which typically have large, complex genomes. The strategy of chromosome walking is based on the assumption that it is difficult and time consuming to find DNA markers that are… CONTINUE READING

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