Chromosome doubling of 2x Solanum species by oryzalin: method development and comparison with spontaneous chromosome doubling in vitro

@article{Chauvin2004ChromosomeDO,
  title={Chromosome doubling of 2x Solanum species by oryzalin: method development and comparison with spontaneous chromosome doubling in vitro},
  author={Jean E. Chauvin and C. Souchet and J. P. Dantec and Daniel Elliss{\`e}che},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={73},
  pages={65-73}
}
A potato breeding scheme implies the possibility of ploidy level manipulation either by reducing the chromosome number of cultivars from 48 to 24 to be able to cross them with diploid related species or by doubling diploid material to reach the generally optimal tetraploid level. In vitro spontaneous chromosome doubling is widely used but can lead to somaclonal variation. Since oryzalin has proven to be efficient as a chromosome doubling agent on potato cell suspension cultures, we tried this… CONTINUE READING

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