Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms


We have investigated DNA segregation in E. coli by inserting multiple lac operator sequences into the chromosome near the origin of replication (oriC), in the hisC gene, a terminus marker, and into plasmids P1 and F. Expression of a GFP-LacI fusion protein allowed visualization of lac operator localization. oriC was shown to be specifically localized at or… (More)
DOI: 10.1016/S0092-8674(00)80377-3

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