Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin, (-)-epigallocatechin gallate (EGCG).

@article{Kusakabe2004ChromosomeAO,
  title={Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin, (-)-epigallocatechin gallate (EGCG).},
  author={Hirokazu Kusakabe and Yujiroh Kamiguchi},
  journal={Mutation research},
  year={2004},
  volume={564 2},
  pages={195-200}
}
The cytogenetic effects of (-)-epigallocatechin gallate (EGCG) on mouse spermatozoa were studied in vitro using an intracytoplasmic sperm injection (ICSI) technique. Spermatozoa were collected by the swim-up method and treated with EGCG at 1 microM and 10 microM. When motile, EGCG-treated spermatozoa were injected into oocytes, structural chromosome aberrations (SCAs) at the first cleavage metaphase did not increase significantly. However, a majority of immotile spermatozoa treated with 10… CONTINUE READING

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Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin , ( -)-epigallocatechin gallate ( EGCG ) .
Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin , ( -)-epigallocatechin gallate ( EGCG ) .
Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin , ( -)-epigallocatechin gallate ( EGCG ) .
However , a majority of immotile spermatozoa treated with 10 microM EGCG had the following abnormalities : pronuclear arrest ( 11% of activated oocytes ) , degenerated sperm chromatin ( chromosome ) mass ( 30% of activated oocytes ) and occurrence of structural chromosome aberrations ( 57% of analyzed metaphases ) .
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