Chromosome aberration induction in Chinese hamster ovary cells by restriction enzymes with different methylation sensitivity.

Abstract

The isoschizomer pair MspI and HpaII were used to investigate whether the putative specificity of restriction endonucleases would be maintained when they were introduced into mammalian cells. Although both enzymes recognize the sequence CCGG, HpaII will cut only if the internal cytosine is unmethylated, whereas MspI will cut regardless of the methylation… (More)

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Cite this paper

@article{Winegar1990ChromosomeAI, title={Chromosome aberration induction in Chinese hamster ovary cells by restriction enzymes with different methylation sensitivity.}, author={Richard A. Winegar and J. W. Phillips and Louise H. Lutze and Wynne Morgan}, journal={Somatic cell and molecular genetics}, year={1990}, volume={16 3}, pages={251-6} }