Chromosome 5 allelic losses are early events in tumours of the papilla of Vater and occur at sites similar to those of gastric cancer.

Abstract

During our studies of DNA fingerprinting of tumours of the pancreas and papilla (ampulla) of Vater, using arbitrarily primed polymerase chain reaction (AP-PCR), we noticed two bands showing a decreased intensity in six of ten ampullary tumours with respect to matched normal tissues. Those bands were both assigned to chromosome 5. Such a finding was somewhat in contrast with the reportedly low frequency of APC gene mutations in ampullary cancers, located at chromosome 5q21, and suggested that loci different from that of APC might be the target of chromosome 5 allelic losses (LOH) in these tumours. Therefore, we analysed chromosome 5 LOH in a panel of 27 ampullary tumours, including eight adenomas, four early- and 15 advanced-stage cancers, using 16 PCR-amplified CA microsatellite polymorphic markers spanning the entire chromosome. Nineteen cases (70%) showed LOH, and the interstitial deletions found in these tumours described two smallest common deleted regions, in which putative suppressor genes might reside. They were at 5q13.3-q14 and at 5q23-q31 respectively, which correspond to those found in gastric tumours. In addition, the presence of 5q LOH in six of eight adenomas and in three of four early-stage cancers suggests that such phenomena occur at early stages of neoplastic progression of the ampullary epithelium.

DOI: 10.1038/bjc.1998.738

Extracted Key Phrases

8 Figures and Tables

Statistics

0200400600'01'03'05'07'09'11'13'15'17
Citations per Year

1,061 Citations

Semantic Scholar estimates that this publication has 1,061 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Achille1998Chromosome5A, title={Chromosome 5 allelic losses are early events in tumours of the papilla of Vater and occur at sites similar to those of gastric cancer.}, author={Alberto Achille and Antonela Bar{\'o}n and Giuseppe Zamboni and Chiara Di Pace and Simonetta Orlandini and Aldo Scarpa}, journal={British Journal of Cancer}, year={1998}, volume={78}, pages={1653 - 1660} }