Chromosomal transfer and in vivo cloning of genes in Vibrio cholerae using RP4::mini-Mu.

@article{Srivastava1989ChromosomalTA,
  title={Chromosomal transfer and in vivo cloning of genes in Vibrio cholerae using RP4::mini-Mu.},
  author={Ranjana Srivastava and V. R. Sinha and Brahm S. Srivastava},
  journal={Gene},
  year={1989},
  volume={75 2},
  pages={253-9}
}
The RP4::mini-Mu replicon has been used to transfer chromosomal genes by conjugation and to clone in vivo metabolic, toxin and flagellar genes of Vibrio cholerae. RP4::mini-Mu was introduced into several strains of V. cholerae and these strains were mated with V. cholerae or Escherichia coli K-12 recipients. R'-episomes carrying the respective genes were… CONTINUE READING