Chromosomal location of TOL plasmid DNA in Pseudomonas putida.

  title={Chromosomal location of TOL plasmid DNA in Pseudomonas putida.},
  author={Martha I. Sinclair and Peter C. Maxwell and Bruce R. Lyon and Bruce W. Holloway},
  journal={Journal of bacteriology},
  volume={168 3},
The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18… CONTINUE READING

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