Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51

@article{Desfarges2006ChromosomalIO,
  title={Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51},
  author={S{\'e}bastien Desfarges and Joseph San Filippo and Marjorie Fournier and Christina Calmels and Anne Caumont-Sarcos and Simon Litvak and Patricia Sung and Vincent Parissi},
  journal={Nucleic Acids Research},
  year={2006},
  volume={34},
  pages={6215 - 6224}
}
HIV-1 integrase (IN) is the key enzyme catalyzing the proviral DNA integration step. Although the enzyme catalyzes the integration step accurately in vitro, whether IN is sufficient for in vivo integration and how it interacts with the cellular machinery remains unclear. We set up a yeast cellular integration system where integrase was expressed as the sole HIV-1 protein and targeted the chromosomes. In this simple eukaryotic model, integrase is necessary and sufficient for the insertion of a… CONTINUE READING

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Evaluation of the activity of HIV-1 integrase over-expressed in eukaryotic cells

B. Van Maele, L. Van Eylen, W. Pluymers, Z. Debyser
Biochem. Biophys. Res. Commun., • 2005
View 2 Excerpts

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