Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed

@article{Bulina2006ChromophoreassistedLI,
  title={Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed},
  author={Maria E Bulina and Konstantin A. Lukyanov and Olga V. Britanova and Daria Onichtchouk and Sergey P. Lukyanov and Dmitriy M Chudakov},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={947-953}
}
The phototoxic red fluorescent GFP-like protein KillerRed has recently been described. The phototoxicity of KillerRed exceeds that of EGFP by at least 1,000-fold, making it the first fully genetically encoded photosensitizer. KillerRed opens up new possibilities for precise light-induced cell killing and target protein inactivation. Because KillerRed is encoded by a gene, it can be expressed in a spatially and temporally regulated manner, under a chosen promoter, and fused with the desired… CONTINUE READING